Active Recombinant HIV-1 (CM244) GP120 protein, His-tagged

• Cat.No.: GP120-1520H
• Product Overview: Recombinant HIV-1 (CM244) GP120 protein(Thr36-Lys511), fused with His tag, was expressed in HEK293.

Specification

• Species: HIV
• Source: HEK293
• Tag: His
• ProteinLength: 36-511 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4.
• Bio-activity: Immobilized Human CD4, Fc Tag at 5 μg/mL (100 μL/well) can bind HIV-1 [HIV-1/Clade E (CM244)] GP120, His Tag with a linear range of 20-156 ng/mL.Immobilized Biotinylated Human CD4, Fc Tag at 1 μg/mL (100 μL/well) can bind HIV-1 [HIV-1/Clade E (CM244)] GP120, His Tag with a linear range of 0.156-0.313 μg/mL.Immobilized Biotinylated Human CD4, Fc Tag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.2ug/well) plate, can bind HIV-1 [HIV-1/Clade E (CM244)] GP120, His Tag with a linear range of 10-78 ng/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus.The protein has a calculated MW of 54.1 kDa. The protein migrates as 65-110 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Thr36-Lys511
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.