Recombinant Hepatitis B Virus C protein, His-tagged

• Cat.No.: C -76H
• Product Overview: The E.coli derived recombinant protein contains the HBV core immunodominant region amino acids 1-186, and fused to a His tag at N- terminus.

Specification

• Species: HBV
• Source: E.coli
• Tag: His
• Protein Length: amino acids 1-186
• Description: Hepatitis B is one of a few known non-retroviralviruses which employ reverse transcriptionas a part of its replication process. (HIV, a completely unrelated virus, also uses reverse transcription, but it is a retrovirus.) HBV invades the cell by binding to surface receptor and become internalized. The viral core particles then migrate to the hepatocyte nucleus and the partially double-stranded, relaxed circular genomes (RC-DNA) are repaired to form a covalently closed circular DNA (cccDNA), which is the template for viral genomic and sub-genomic RNAs by cellular RNA polymerase II. Of these, the pregenomic RNA(pgRNA is selectively packaged into progeny capsids and is then reverse-transcribed into new RC-DNA. The core can either bud into the endoplasmic reticulum to be enveloped or exported from the cell or recycled back into the genome for conversion to cccDNA.
• Form: 25mM Tris-HCl pH-8.0, 1.5 mM EDTA, 1.5mM Urea & 50% glycerol.
• Purity: HBV Core protein is >90% pure as determined by 10% PAGE (Coomassie staining).
• Usage: The products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
• Specificity: Immunoreactive with sera HBV-infected individuals.
• Storage: HBV Core protein although stable at 4°C for 1 week, should be stored below -18°C. Please prevent freeze thaw cycles.
• Shipping: Ice Packs

Gene Information

• Gene Name: C precore; HBe antigen; PreC; HBeAg; precore protein; external core antigen; HBeAg; p25 [ Hepatitis B virus ]
• Official Symbol: C
• Synonyms: C; precore; HBe antigen; PreC; HBeAg; precore protein; external core antigen; HBeAg; p25; pre-capsid protein; C0 uORF; capsid protein
• Gene ID: 944568
• Protein Refseq: YP_009173857.1
• UniProt ID: P03146