Recombinant H3N2 M1 protein(Met1-Lys252), His-tagged

• Cat.No.: M1-426H
• Product Overview: Recombinant influenza A virus (A/Aichi/2/1968(H3N2)) matrix protein 1 (AFM71858.1) (Met1-Lys252), termed as M1, was expressed in E. coli, fused with a N-terminal polyhistidine tag.

Specification

• Source: E. coli
• Species: H3N2
• Tag: His
• Protein length: Met1-Lys252
• Form: Lyophilized from sterile 1mM EDTA, 50mM Tris, 50mM NaCl, 5% glycerol. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant M1 subunit of influenza A H3N2 consists of 268 amino acids and has a predicted molecular mass of 30 kDa. It migrates as an approximately 35 kDa band in SDS-PAGE under reducing conditions.
• Purity: > 85 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.