Recombinant Full Length Mycobacterium Avium (Dimethylallyl)Adenosine Trna Methylthiotransferase Miab Protein, His-Tagged

• Cat.No.: RFL8705MF
• Product Overview: Recombinant Full Length Mycobacterium avium (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB Protein (A0QIR4) (1-715aa), fused to N-terminal His tag, was expressed in E. coli.

Specification

• Species: Mycobacterium avium
• Source: E.coli
• Tag: His
• ProteinLength: Full Length (1-715)
• Form: Lyophilized powder
• AA Sequence: MTCGFSRADRSPYHGPVTSTVARDVSGVRTYQVRTYGCQMNVHDSERLAGLLEAAGYRRA AEGAEVADVVVFNTCAVRENADNKLYGNLSHLAPRKRSNPQMQIAVGGCLAQKDREAVLR RAPWVDVVFGTHNIGSLPTLLERARHNKAAQVEIAEALQQFPSSLPSARESAYAAWVSIS VGCNNSCTFCIVPSLRGKEVDRSPDDILAEVRSLVADGVLEVTLLGQNVNAYGVSFADPA LPRDRGAFARLLRACGEIDGLERVRFTSPHPAEFTDDVIEAMAQTPNVCPALHMPLQSGS DRVLRAMRRSYRAERYLGIIDRVRAAMPHAAITTDLIVGFPGETEEDFAATLDVVRRARF AAAFTFQYSKRPGTPAAELDGQIPKAVVQERYERLVELQESISLQGNQALVGQTVELLVA TGEGRKDSATARMSGRARDGRLVHFAADDRVRPGDLVTTVITGAAPHHLIADAGILSHRR TRAGDAHAPAGARAASVSACPPSGRRRPGPTRWMCLMNDDRNHDDPRLGALRTEIEAAER RVAGGIDPGARGFVVSILVFVLLGSFILPHTGDVRGWDVLFGTHDAGAAAVALPSRVFGW LALVFGVGFSTLALVTRRWALAWIALAGTAIAGAAGMLAIWSRQTVPAGHPGPGWGLIVA WITVLVLIYQWARVVWSRTIVQLAAEEQRRRVAAQQQSTTLLDDLPKPEDPAAGT
• Purity: Greater than 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Tris/PBS-based buffer, 6% Trehalose, pH 8.0
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Gene Information

• Gene Name: miaB
• Synonyms: miaB; MAV_3625; tRNA-2-methylthio-N(6-dimethylallyladenosine synthase; (Dimethylallyladenosine tRNA methylthiotransferase MiaB; tRNA-i(6A37 methylthiotransferase
• UniProt ID: A0QIR4