Recombinant Full Length Mouse GDP-mannose pyrophosphorylase B Protein, Flag tagged

• Cat.No.: Gmppb-30MFL
• Product Overview: Purified recombinant protein of Mouse GDP-mannose pyrophosphorylase B (Gmppb), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Predicted to enable mannose-1-phosphate guanylyltransferase activity. Predicted to be involved in GDP-mannose biosynthetic process; cell wall mannoprotein biosynthetic process; and protein glycosylation. Located in mitochondrion. Is expressed in several structures, including alimentary system; genitourinary system; nervous system; respiratory system; and sensory organ. Human ortholog(s) of this gene implicated in autosomal recessive limb-girdle muscular dystrophy type 2T; congenital muscular dystrophy-dystroglycanopathy A14; and muscular dystrophy-dystroglycanopathy. Orthologous to human GMPPB (GDP-mannose pyrophosphorylase B).
• Tag: C-Flag
• Protein length: 1-360aa
• Molecular Mass: 39.9 kDa
• AA Sequence: MKALILVGGYGTRLRPLTLSTPKPLVDFCNKPILLHQVEALAAAGVDHVILAVSYMSQMLEKEMKAQEQRLGIRISMSHEEEPLGTAGPLALARDLLSETADPFFVLNSDVICDFPFQAMVQFHRHHGQEGSILVTKVEEPSKYGVVVCEADTGRIHRFVEKPQVFVSNKINAGMYILSPAVLQRIQLKPTSIEKEIFPVMAKEGQLYAMELQGFWMDIGQPKDFLTGMCLFLQSLRQKHPERLYSGPGIVGNVLVDPSARIGQNCSIGPNVSLGPGVVVEDGVCIRRCTVLRDAHIRSHSWLESCIVGWRCRVGQWVRMENVTVLGEDVIVNDELYLNGASVLPHKSIGESVPEPRIIMTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Notes: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Storage Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
• Concentration: > 0.05 μg/μL as determined by microplate BCA method

Gene Information

• Gene Name: Gmppb GDP-mannose pyrophosphorylase B [ Mus musculus (house mouse) ]
• Official Symbol: Gmppb
• Synonyms: Gmppb; GDP-mannose pyrophosphorylase B; mannose-1-phosphate guanyltransferase beta; GTP-mannose-1-phosphate guanylyltransferase beta; EC 2.7.7.13; E430010H19
• Gene ID: 331026
• mRNA Refseq: NM_177910
• Protein Refseq: NP_808578
• UniProt ID: Q8BTZ7