Recombinant Full Length Human Ubiquitin Protein, Propargylamide Labeled

• Cat.No.: Ubiquitin-15HFL
• Product Overview: This product consists of a full-length human, mature ubiquitin polypeptide (amino acids 1-76), expressed in E. coli with a c-terminal warhead (Propargylamide).

Specification

• Description: Ubiquitin is a small (8.5 kDa) regulatory protein that has been found in almost all tissues of eukaryotic organisms. The addition of ubiquitin to a substrate protein is called ubiquitination or ubiquitylation. Ubiquitination can affect proteins in many ways; it can signal for their degradation via the proteasome, alter their cellular location, affect their activity, and promote or prevent protein interactions. Removal of ubiquitin from a substrate protein occurs via deconjugating enzymes, of which there are nearly 100 known enzymes with various linkage specificities. Ubiquitin-Propargylamide is a potent, irreversible, and specific inhibitor of deubiquitinating enzymes (DUBs) based on a C-terminal electrophilic Propargylamide group. It targets three of the four major DUB families: UCH (Ubiquitin c-terminal hydrolases), USP (Ubiquitin Specific Proteases), OTU (Ovarian Tumor Proteases), and MJD (Machado-Josephin domain proteases) while JAMM metalloproteases are not inhibited.
• Source: E. coli
• Species: Human
• Protein length: 1-76aa
• Conjugation/Label: Propargylamide
• Molecular Mass: 8.6 kDa
• Purity: >97% by LCMS
• Usage: Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human Ubiquitin Propargylamide concentration of 1-5 μM.
• Applications: Ub-Propargylamide can be used for activity profiling experiments and determining DUB inhibitor specificity.
• Notes: For Research Use Only, Not For Use In Humans.
• Storage: Store at −80 centigrade after product arrival. Avoid multiple freeze/thaws. It is recommended to make multiple aliquots after the first thaw.
• Storage Buffer: 50mM MES pH 6.0, 100mM NaCl
• Concentration: 300 μM, 2.6 mg/mL
• Substrate Properties: Protein-Based Substrate. Typical experimental concentration 1-5 μM.