Recombinant Full Length human TKT protein,His-tagged

• Cat.No.: TKT-12HFL
• Product Overview: Recombinant Full Length human TKT protein,His-tagged was expressed in E. coli.

Specification

• Description: TKT, also known as transketolase, is a thiamine-dependent enzyme which plays a role in the channeling of excess sugar phosphates to glycolysis in the pentose phosphate pathway. Multiple alternatively spliced variants,encoding the same protein, have been identified. Recombinant human TKT protein, fused to His-tag at Nterminus, was expressed in E. coli and purified by using conventional chromatography techniqu.
• Source: E.coli
• Species: Human
• Tag: His
• Protein length: 1-623 aa
• Molecular Mass: 70 kDa
• AA Sequence: MESYHKPDQQ KLQALKDTAN RLRISSIQAT TAAGSGHPTS CCSAAEIMAVLFFHTMRYKS QDPRNPHNDR FVLSKGHAAP ILYAVWAEAG FLAEAELLNL RKISSDLDGH PVPKQAFTDV ATGSLGQGLG
  AACGMAYTGK YFDKASYRVY CLLGDGELSE GSVWEAMAFA SIYKLDNLVA ILDINRLGQS DPAPLQHQMD IYQKRCEAFG
  WHAIIVDGHS VEELCKAFGQ AKHQPTAIIA KTFKGRGITG VEDKESWHGK PLPKNMAEQI IQEIYSQIQS KKKILATPPQ
  EDAPSVDIAN IRMPSLPSYK VGDKIATRKA YGQALAKLGH ASDRIIALDG DTKNSTFSEI FKKEHPDRFI ECYIAEQNMV
  SIAVGCATRN RTVPFCSTFA AFFTRAFDQI RMAAISESNI NLCGSHCGVS IGEDGPSQMA LEDLAMFRSV PTSTVFYPSD
  GVATEKAVEL AANTKGICFI RTSRPENAII YNNNEDFQVG QAKVVLKSKD DQVTVIGAGV TLHEALAAAE LLKKEKINIR
  VLDPFTIKPL DRKLILDSAR ATKGRILTVE DHYYEGGIGE AVSSAVVGEP GITVTHLAVN RVPRSGKPAE LLKMFGIDRD
  AIAQAVRGLI TKA
• Purity: > 85% by SDS-PAGE
• Applications: SDS-PAGE
• Notes: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.
• Storage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25mg/ml (determined by Bradford assay)
• Storage Buffer: Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.1M Nacl, 1mM DTT
• References: Choudhary C. et al. (2009) Science 325:834-840.

Gene Information

• Gene Name: TKT transketolase [ Homo sapiens (human) ]
• Official Symbol: TKT
• Synonyms: Transketolase, TK, TKT1,TKT
• Gene ID: 7086
• mRNA Refseq: NM_001064
• Protein Refseq: NP_001055
• UniProt ID: P29401