Recombinant Full Length Human RAPSN Protein, His-tagged

• Cat.No.: RAPSN-322H
• Product Overview: RAPSN, 1-412aa, Mouse, His tag, E.coli

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-412 a.a.
• Description: 43 kDa receptor-associated protein of the synapse, also known as RAPSN, is expressed in the postsynaptic membrane of skeletal muscle. RAPSN is required for the clustering of nicotinic acetylcholine receptors (nAChR). It self-associates through at least two of its seven tetra-tricopeptide repeats (TPRs). RAPSN interacts with the large intracellular domain of the nAChR a subunit through the hydrophobic surface of the coiled-coil domain. This protein modifies trafficking of AChR within the cell. Overexpression inhibits agrin-induced AChR clustering pathway. Recombinant human RAPSN protein, fused to His-tag at N-terminus, was expressed in E.coli.
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol 0.4M Urea
• Molecular Mass: 48.8 kDa (435aa)
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMGQDQTK QQIEKGLQLY QSNQTEKALQ VWMKVLEKGS DLVGRFRVLG CLVTAHSEMG RYKEMLKFAV VQIDTARGLE DADFLLESYL NLARSNEKLC EFHKTISYCK TCLGLPGTRA GAQLGGQVSL SMGNAFLGLS LFQKALESFE KALRYAHNND DTMLECRVCC SLGSFYAQVK DYEKALFFPC KAAELVNDYG KGWSLKYRAM SQYHMAVAYR LLGHLGSAME CCEESMKIAL QHGDRPLQAL CLLCFADIHR SRGDLETAFP RYDSAMSIMT EIGNRLGQVH VLLGVAKCWM ARKVQDKALD AIEKAQDLAE EVGNKLSQLK LHCLSESIYR SKGLQRELRT HVVRFHECVE ETELYCGLCG ESIGERNSRL QALPCSHIFH LRCLQNNGTR SCPNCRRSSM KPGFV
• Purity: >85% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: RAPSN receptor-associated protein of the synapse [ Homo sapiens ]
• Official Symbol: RAPSN
• Synonyms: RAPSN; receptor-associated protein of the synapse; receptor associated protein of the synapse, 43kD; 43 kDa receptor-associated protein of the synapse; CMS1D; CMS1E; rapsyn; RNF205; RING finger protein 205; 43 kda postsynaptic protein; acetylcholine receptor-associated 43 kda protein; RAPSYN; MGC3597
• Gene ID: 5913
• mRNA Refseq: NM_005055
• Protein Refseq: NP_005046
• MIM: 601592
• UniProt ID: Q13702
• Chromosome Location: 11p11.2
• Function: acetylcholine receptor binding; binding; metal ion binding; zinc ion binding