Recombinant Full Length Human Prostaglandin Reductase 1 / PTGR1, His-tagged

• Cat.No.: PTGR1-2520H
• Product Overview: Recombinant human PTGR1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-329aa
• Description: PTGR1 belongs to the NADP-dependent oxidoreductase L4BD family. This enzyme catalyzes the conversion of leukotriene B4 into its biologically less active metabolite, 12-oxo-leukotriene B4. This is an initial and key step of metabolic inactivation of leukotriene B4. It is highly expressed in the kidney, liver, and intestine but not in leukocytes.
• Form: Liquid. In 20 mM Tris-HCl buffer, pH7.5, 10% glycerol, 1mM DTT, 200mM NaCl.
• Molecular Mass: 38.6 kDa (354aa), confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSEFMVRTK TWTLKKHFVG YPTNSDFELK TAELPPLKNG EVLLEALFLT VDPYMRVAAK RLKEGDTMMG QQVAKVVESK NVALPKGTIV LASPGWTTHS ISDGKDLEKL LTEWPDTIPL SLALGTVGMP GLTAYFGLLE ICGVKGGETV MVNAAAGAVG SVVGQIAKLK GCKVVGAVGS DEKVAYLQKL GFDVVFNYKT VESLEETLKK ASPDGYDCYF DNVGGEFSNT VIGQMKKFGR IAICGAISTY NRTGPLPPGP PPEIVIYQEL RMEAFVVYRW QGDARQKALK DLLKWVLEGK IQYKEYIIEG FENMPAAFMG MLKGDNLGKT IVKA
• Purity: >90% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: PTGR1 prostaglandin reductase 1 [ Homo sapiens ]
• Official Symbol: PTGR1
• Synonyms: PTGR1; prostaglandin reductase 1; leukotriene B4 12 hydroxydehydrogenase , LTB4DH; ZADH3; zinc binding alcohol dehydrogenase domain containing 3; PRG-1; 15-oxoprostaglandin 13-reductase; leukotriene B4 12-hydroxydehydrogenase; NADP-dependent leukotriene B4 12-hydroxydehydrogenase; PGR1; LTB4DH; FLJ99229; MGC34943
• Gene ID: 22949
• mRNA Refseq: NM_001146108
• Protein Refseq: NP_001139580
• MIM: 601274
• UniProt ID: Q14914
• Chromosome Location: 9q32
• Function: 15-oxoprostaglandin 13-oxidase activity; 2-alkenal reductase [NAD(P)] activity; alcohol dehydrogenase (NAD) activity; nucleotide binding; oxidoreductase activity; zinc ion binding