Recombinant Full Length Human presenilin 1 Protein, Flag tagged

• Cat.No.: PSEN1-23HFL
• Product Overview: Recombinant protein of human presenilin 1 (PSEN1), transcript variant 1 with C-Myc/DDK tag was expressed in HEK293.

Specification

• Description: Alzheimer's disease (AD) patients with an inherited form of the disease carry mutations in the presenilin proteins (PSEN1; PSEN2) or in the amyloid precursor protein (APP). These disease-linked mutations result in increased production of the longer form of amyloid-beta (main component of amyloid deposits found in AD brains). Presenilins are postulated to regulate APP processing through their effects on gamma-secretase, an enzyme that cleaves APP. Also, it is thought that the presenilins are involved in the cleavage of the Notch receptor, such that they either directly regulate gamma-secretase activity or themselves are protease enzymes. Several alternatively spliced transcript variants encoding different isoforms have been identified for this gene, the full-length nature of only some have been determined.
• Source: HEK293
• Species: Human
• Tag: C-Flag
• Protein length: 1-467aa
• Molecular Mass: 52.5 kDa
• AA Sequence: MTELPAPLSYFQNAQMSEDNHLSNTVRSQNDNRERQEHNDRRSLGHPEPLSNGRPQGNSRQVVEQDEEEDEELTLKYGAKHVIMLFVPVTLCMVVVVATIKSVSFYTRKDGQLIYTPFTEDTETVGQRALHSILNAAIMISVIVVMTILLVVLYKYRCYKVIHAWLIISSLLLLFFFSFIYLGEVFKTYNVAVDYITVALLIWNFGVVGMISIHWKGPLRLQQAYLIMISALMALVFIKYLPEWTAWLILAVISVYDLVAVLCPKGPLRMLVETAQERNETLFPALIYSSTMVWLVNMAEGDPEAQRRVSKNSKYNAESTERESQDTVAENDDGGFSEEWEAQRDSHLGPHRSTPESRAAVQELSSSILAGEDPEERGVKLGLGDFIFYSVLVGKASATASGDWNTTIACFVAILIGLCLTLLLLAIFKKALPALPISITFGLVFYFATDYLVQPFMDQLAFHQFYITRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: > 0.05 μg/μL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
• Notes: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.

Gene Information

• Gene Name: PSEN1 presenilin 1 [ Homo sapiens (human) ]
• Official Symbol: PSEN1
• Synonyms: PSEN1; presenilin 1; AD3; FAD; PS1; PS-1; S182; PSNL1; ACNINV3; presenilin-1; familial Alzheimer Disease; EC 3.4.23.-
• Gene ID: 5663
• mRNA Refseq: NM_000021
• Protein Refseq: NP_000012
• MIM: 104311
• UniProt ID: P49768