Recombinant Full Length Human MRPL43 Protein, GST-tagged

• Cat.No.: MRPL43-6423HF
• Product Overview: Human MRPL43 full-length ORF ( NP_115488.2, 1 a.a. - 159 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. This gene and the gene for a semaphorin class 4 protein (SEMA4G) overlap at map location 10q24.31 and are transcribed in opposite directions. Sequence analysis identified multiple transcript variants encoding at least four different protein isoforms. [provided by RefSeq
• Source: In Vitro Cell Free System
• Species: Human
• Tag: GST
• Molecular Mass: 44.3 kDa
• Protein length: 159 amino acids
• AA Sequence: MTARGTPSRFLASVLHNGLGRYVQQLQRLSFSVSRDGASSRGAREFVEREVIDFARRNPGVVIYVNSRPCCVPRVVAEYLNGAVREESIHCKSVEEISTLVQKLADQSGLDVIRIRKPFHTDNPSIQGQWHPFTNKPTTFRGLRPREVQDPAPAQVQAQ
• Applications: Enzyme-linked Immunoabsorbent Assay
  Western Blot (Recombinant protein)
  Antibody Production
  Protein Array
• Notes: Best use within three months from the date of receipt of this protein.
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCl, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: MRPL43 mitochondrial ribosomal protein L43 [ Homo sapiens ]
• Official Symbol: MRPL43
• Synonyms: MRPL43; mitochondrial ribosomal protein L43; 39S ribosomal protein L43, mitochondrial; bMRP36a; L43mt; MRP-L43; mitochondrial ribosomal protein bMRP36a; MGC17989; MGC48892
• Gene ID: 84545
• mRNA Refseq: NM_032112
• Protein Refseq: NP_115488
• MIM: 611848
• UniProt ID: Q8N983