Recombinant Human MRPL43 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: MRPL43-6638H
• Product Overview: MRPL43 MS Standard C13 and N15-labeled recombinant protein (NP_789762) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. This gene and the gene for a semaphorin class 4 protein (SEMA4G) overlap at map location 10q24.31 and are transcribed in opposite directions. Sequence analysis identified multiple transcript variants encoding at least four different protein isoforms.
• Source: HEK293
• Species: Human
• Tag: Myc&DDK
• Molecular Mass: 23.4 kDa
• AA Sequence: MTARGTPSRFLASVLHNGLGRYVQQLQRLSFSVSRDGASSRGAREFVEREVIDFARRNPGVVIYVNSRPCCVPRVVAEYLNGAVREESIHCKSVEEISTLVQKLADQSGLDVIRIRKPFHTDNPSIQGQWHPFTNKPTTFRGLRPREVQDPAPAQDTGLRLSAVAPQILLPGWPDPPDLPTVDPISSSLTSAPAPMLSAVSCLPIVPALTTVCSATRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: MRPL43 mitochondrial ribosomal protein L43 [ Homo sapiens (human) ]
• Official Symbol: MRPL43
• Synonyms: MRPL43; mitochondrial ribosomal protein L43; 39S ribosomal protein L43, mitochondrial; bMRP36a; L43mt; MRP-L43; mitochondrial ribosomal protein bMRP36a; MGC17989; MGC48892
• Gene ID: 84545
• mRNA Refseq: NM_176792
• Protein Refseq: NP_789762
• MIM: 611848
• UniProt ID: Q8N983