Recombinant Full Length Human LRP5 Protein, C-Flag-tagged

• Cat.No.: LRP5-245HFL
• Product Overview: Recombinant Full Length Human LRP5 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a transmembrane low-density lipoprotein receptor that binds and internalizes ligands in the process of receptor-mediated endocytosis. This protein also acts as a co-receptor with Frizzled protein family members for transducing signals by Wnt proteins and was originally cloned on the basis of its association with type 1 diabetes mellitus in humans. This protein plays a key role in skeletal homeostasis and many bone density related diseases are caused by mutations in this gene. Mutations in this gene also cause familial exudative vitreoretinopathy. Alternative splicing results in multiple transcript variants.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 179 kDa
• AA Sequence: MEAAPPGPPWPLLLLLLLLLALCGC PAPAAASPLLLFANRRDVRLVDAGG VKLESTIVVSGLEDAAAVDF QFSKGAVYWTDVSEEAIKQTYLNQT GAAVQNVVISGLVSPDGLACDWVGK KLYWTDSETNRIEVANLNGT SRKVLFWQDLDQPRAIALDPAHGYM YWTDWGETPRIERAGMDGSTRKIIV DSDIYWPNGLTIDLEEQKLY WADAKLSFIHRANLDGSFRQKVVEG SLTHPFALTLSGDTLYWTDWQTRSI HACNKRTGGKRKEILSALYS PMDIQVLSQERQPFFHTRCEEDNGG CSHLCLLSPSEPFYTCACPTGVQLQ DNGRTCKAGAEEVLLLARRT DLRRISLDTPDFTDIVLQVDDIRHA IAIDYDPLEGYVYWTDDEVRAIRRA YLDGSGAQTLVNTEINDPDG IAVDWVARNLYWTDTGTDRIEVTRL NGTSRKILVSEDLDEPRAIALHPVM GLMYWTDWGENPKIECANLD GQERRVLVNASLGWPNGLALDLQEG KLYWGDAKTDKIEVINVDGTKRRTL LEDKLPHIFGFTLLGDFIYW TDWQRRSIERVHKVKASRDVIIDQL PDLMGLKAVNVAKVVGTNPCADRNG GCSHLCFFTPHATRCGCPIG LELLSDMKTCIVPEAFLVFTSRAAI HRISLETNNNDVAIPLTGVKEASAL DFDVSNNHIYWTDVSLKTIS RAFMNGSSVEHVVEFGLDYPEGMAV DWMGKNLYWADTGTNRIEVARLDGQ FRQVLVWRDLDNPRSLALDP TKGYIYWTEWGGKPRIVRAFMDGTN CMTLVDKVGRANDLTIDYADQRLYW TDLDTNMIESSNMLGQERVV IADDLPHPFGLTQYSDYIYWTDWNL HSIERADKTSGRNRTLIQGHLDFVM DILVFHSSRQDGLNDCMHNN GQCGQLCLAIPGGHRCGCASHYTLD PSSRNCSPPTTFLLFSQKSAISRMI PDDQHSPDLILPLHGLRNVK AIDYDPLDKFIYWVDGRQNIKRAKD DGTQPFVLTSLSQGQNPDRQPHDLS IDIYSRTLFWTCEATNTINV HRLSGEAMGVVLRGDRDKPRAIVVN AERGYLYFTNMQDRAAKIERAALDG TEREVLFTTGLIRPVALVVD NTLGKLFWVDADLKRIESCDLSGAN RLTLEDANIVQPLGLTILGKHLYWI DRQQQMIERVEKTTGDKRTR IQGRVAHLTGIHAVEEVSLEEFSAH PCARDNGGCSHICIAKGDGTPRCSC PVHLVLLQNLLTCGEPPTCS PDQFACATGEIDCIPGAWRCDGFPE CDDQSDEEGCPVCSAAQFPCARGQC VDLRLRCDGEADCQDRSDEV DCDAICLPNQFRCASGQCVLIKQQC DSFPDCIDGSDELMCEITKPPSDDS PAHSSAIGPVIGIILSLFVM GGVYFVCQRVVCQRYAGANGPFPHE YVSGTPHVPLNFIAPGGSQHGPFTG IACGKSMMSSVSLMGGRGGV PLYDRNHVTGASSSSSSSTKATLYP PILNPPPSPATDPSLYNMDMFYSSN IPATARPYRPYIIRGMAPPT TPCSTDVCDSDYSASRWKASKYYLD LNSDSDPYPPPPTPHSQYLSAEDSC PPSPATERSYFHLFPPPPSP
  CTDSSTRTRPLEQKLISEEDLAAND ILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Wnt signaling pathway

Gene Information

• Gene Name: LRP5 LDL receptor related protein 5 [ Homo sapiens (human) ]
• Official Symbol: LRP5
• Synonyms: HBM; LR3; OPS; EVR1; EVR4; LRP7; OPPG; BMND1; LRP-5; LRP-7; OPTA1; PCLD4; VBCH2
• Gene ID: 4041
• mRNA Refseq: NM_002335.4
• Protein Refseq: NP_002326.2
• MIM: 603506
• UniProt ID: O75197