Recombinant Full Length Human HGS Protein, C-Flag-tagged

• Cat.No.: HGS-1993HFL
• Product Overview: Recombinant Full Length Human HGS Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene regulates endosomal sorting and plays a critical role in the recycling and degradation of membrane receptors. The encoded protein sorts monoubiquitinated membrane proteins into the multivesicular body, targeting these proteins for lysosome-dependent degradation.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 86 kDa
• AA Sequence: MGRGSGTFERLLDKATSQLLLETDW ESILQICDLIRQGDTQAKYAVNSIK KKVNDKNPHVALYALEVMES VVKNCGQTVHDEVANKQTMEELKDL LKRQVEVNVRNKILYLIQAWAHAFR NEPKYKVVQDTYQIMKVEGH VFPEFKESDAMFAAERAPDWVDAEE CHRCRVQFGVMTRKHHCRACGQIFC GKCSSKYSTIPKFGIEKEVR VCEPCYEQLNRKAEGKATSTTELPP EYLTSPLSQQSQLPPKRDETALQEE EELQLALALSQSEAEEKERL RQKSTYTSYPKAEPMPSASSAPPAS SLYSSPVNSSAPLAEDIDPELARYL NRNYWEKKQEEARKSPTPSA PVPLTEPAAQPGEGHAAPTNVVENP LPETDSQPIPPSGGPFSEPQFHNGE SEESHEQFLKALQNAVTTFV NRMKSNHMRGRSITNDSAVLSLFQS INGMHPQLLELLNQLDERRLYYEGL QDKLAQIRDARGALSALREE HREKLRRAAEEAERQRQIQLAQKLE IMRQKKQEYLEVQRQLAIQRLQEQE KERQMRLEQQKQTVQMRAQM PAFPLPYAQLQAMPAAGGVLYQPSG PASFPSTFSPAGSVEGSPMHGVYMS QPAPAAGPYPSMPSTAADPS MVSAYMYPAGATGAQAAPQAQAGPT ASPAYSSYQPTPTAGYQNVASQAPQ SLPAISQPPQSSTMGYMGSQ SVSMGYQPYNMQNLMTTLPSQDASL PPQQPYIAGQQPMYQQMAPSGGPPQ QQPPVAQQPQAQGPPAQGSE AQLISFD TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome
• Protein Pathways: Endocytosis

Gene Information

• Gene Name: HGS hepatocyte growth factor-regulated tyrosine kinase substrate [ Homo sapiens (human) ]
• Official Symbol: HGS
• Synonyms: HRS
• Gene ID: 9146
• mRNA Refseq: NM_004712.5
• Protein Refseq: NP_004703.1
• MIM: 604375
• UniProt ID: O14964

Reviews

05/13/2019

Minimal background noise in our assays was indicative of the product's superior quality.

01/24/2018

Compatibility with a range of assays expanded our research possibilities significantly.

12/19/2016

Our research benefited greatly from the product's ability to induce specific cellular responses, enriching our findings.

Q&As

How does HGS contribute to the regulation of cell signaling and receptor downregulation in the endocytic pathway?

HGS contributes to the regulation of cell signaling by promoting the downregulation of activated receptors in the endocytic pathway.

What diseases or conditions are associated with dysregulation or abnormal expression of HGS?

Dysregulation or abnormal expression of HGS is associated with conditions such as cancer and neurodegenerative disorders.

How does HGS participate in the endocytic pathway, particularly in the degradation of receptor tyrosine kinases (RTKs)?

HGS participates in the endocytic pathway by facilitating the degradation of receptor tyrosine kinases (RTKs) through the ESCRT complex.

What is the primary function of Hepatocyte growth factor-regulated tyrosine kinase substrate (HGS) in cellular processes and intracellular trafficking?

Hepatocyte growth factor-regulated tyrosine kinase substrate (HGS) functions in cellular processes, primarily playing a role in intracellular trafficking.

In which cellular compartments is HGS predominantly localized?

HGS is predominantly localized in endosomes and lysosomes, key compartments for the endocytic pathway.

Are there specific interactions or binding partners of HGS that influence its role in intracellular trafficking?

Specific interactions or binding partners of HGS include ESCRT components, influencing its role in receptor downregulation and intracellular trafficking.

Are there therapeutic implications or potential targets related to HGS in diseases involving intracellular trafficking and signaling?

There are potential therapeutic implications related to HGS in diseases involving intracellular trafficking and signaling, making it a target for drug development.