Recombinant Full Length Human growth arrest and DNA-damage-inducible, alpha Protein, His&Flag&StrepII tagged

• Cat.No.: GADD45A-146H
• Product Overview: Recombinant human GADD45A protein (P24522) (Met 1-Arg 165) with a Twin strep tag at the N-terminus and both His, Flag tag at the C-terminus was expressed in E. coli.

Specification

• Description: GADD45 alpha (Growth arrest and DNA damage-inducible protein 45 alpha) is a 19 kDa member of the GADD45 family of proteins. It is ubiquitously expressed, and induced by DNA damage. Upon induction, GADD45 alpha binds to PCNA, stimulates DNA excision repair, and initiates G2 cell cycle arrest. Human GADD45 alpha is 165 amino acids (aa) in length. It is reported to dimerize with GADD45-beta and -gamma, and to homo-dimerize, -trimerize and -tetramerize via residues 33‑61 and 133‑165. There is one splice variant that shows a Ser substitution for aa 14‑49. GADD45 alpha is 55% aa identical to GADD45-beta and -gamma, and shares 94% aa identity with mouse GADD45 alpha.
• Source: E. coli
• Species: Human
• Tag: N-Twin strep and C-His&Flag
• Protein length: 1-165aa
• Molecular Mass: 25 kDa
• Endotoxin: Less than 1.0 EU/μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade. It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.
• Reconstitution: Reconstitute at 250 μg/mL in sterile water.

Gene Information

• Gene Name: GADD45A growth arrest and DNA-damage-inducible, alpha [ Homo sapiens (human) ]
• Official Symbol: GADD45A
• Synonyms: GADD45A; growth arrest and DNA-damage-inducible, alpha; DDIT1; growth arrest and DNA damage-inducible protein GADD45 alpha; GADD45; DDIT-1; DNA damage-inducible transcript-1; DNA-damage-inducible transcript 1; DNA damage-inducible transcript 1 protein; growth arrest and DNA-damage-inducible 45 alpha
• Gene ID: 1647
• mRNA Refseq: NM_001199741
• Protein Refseq: NP_001186670
• MIM: 126335
• UniProt ID: P24522