Active Recombinant Human CASP3 Protein, 29-277aa, C-His tagged

• Cat.No.: CASP3-13HFL
• Product Overview: Recombinant Human Caspase-3, fused to Histag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: Insect cells
• Tag: His
• Protein Length: 29-277aa
• Description: Caspase-3, also known as caspase-3 isoform a, is a member of the cysteine-aspartic acid protease (caspase) family of enzymes. This protein is most abundant in cell lines of lymphocytic origin. Also, it activates caspases 6 and 7 and the protein itself is processed and is activated by caspases 8, 9, and 10. Caspases exist as inactive proenzymes that undergo proteolytic processing at conserved aspartic residues to produce two subunits that dimerize to form the active enzyme. Members of the caspase family of proteases play central roles in coordinating the stereotypical events that occur during apoptosis.
• Form: Liquid
• Bio-activity: > 5,000 pmol/min/μg. One unit will liberate 1pmole of Ac-DEVD-AFC to Ac-DEVD and AFC per minute at pH7.5 at 25 centigrade.
• Molecular Mass: 29.4 kDa (256aa)
• AA Sequence: MSGISLDNSYKMDYPEMGLCIIINNKNFHKSTGMTSRSGTDVDAANLRETFRNLKYEVRNKNDLTREEIVELMRDVSKEDHSKRSSFVCVLLSHGEEGIIFGTNGPVDLKKITNFFRGDRCRSLTGKPKLFIIQACRGTELDCGIETDSGVDDDMACHKIPVEADFLYAYSTAPGYYSWRNSKDGSWFIQSLCAMLKQYADKLEFMHILTRVNRKVATEFESFSFDATFHAKKQIPCIVSMLTKELYFYH
• Endotoxin: < 1 EU/μg of protein (determined by LAL method)
• Purity: > 90% by SDS-PAGE
• Applications: SDS-PAGE, Enzyme Activity
• Notes: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.
• Storage: Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/mL (determined by absorbance at 280nm)
• Storage Buffer: 20mM HEPES buffer (pH 7.4) containing 0.1M NaCl, 1mM EDTA, 1mM DTT, 20% glycerol
• GeneID: 836
• References: 1. Walsh JG., et al, (2008) Proc. Natl. Scad. Sci. USA 105:12815.
  2. Fernandes-Alnemri T., et al, (1994) J. Biol. Chem. 269:30761-30764.

Gene Information

• Gene Name: CASP3 caspase 3 [ Homo sapiens (human) ]
• Official Symbol: CASP3
• Synonyms: CASP3; caspase 3; CPP32; SCA-1; CPP32B; caspase-3; CASP-3; CPP-32; PARP cleavage protease; SREBP cleavage activity 1; apopain; caspase 3, apoptosis-related cysteine peptidase; caspase 3, apoptosis-related cysteine protease; cysteine protease CPP32; procaspase3; protein Yama; EC 3.4.22.56
• mRNA Refseq: NM_032991
• Protein Refseq: NP_116786
• MIM: 600636
• UniProt ID: P42574