Recombinant Full Length Human ASPH Protein, GST-tagged

• Cat.No.: ASPH-1057HF
• Product Overview: Human ASPH full-length ORF ( NP_115856.1, 1 a.a. - 210 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: This gene is thought to play an important role in calcium homeostasis. The gene is expressed from two promoters and undergoes extensive alternative splicing. The encoded set of proteins share varying amounts of overlap near their N-termini but have substantial variations in their C-terminal domains resulting in distinct functional properties. The longest isoforms (a and f) include a C-terminal Aspartyl/Asparaginyl beta-hydroxylase domain that hydroxylates aspartic acid or asparagine residues in the epidermal growth factor (EGF)-like domains of some proteins, including protein C, coagulation factors VII, IX, and X, and the complement factors C1R and C1S. Other isoforms differ primarily in the C-terminal sequence and lack the hydroxylase domain, and some have been localized to the endoplasmic and sarcoplasmic reticulum. Some of these isoforms are found in complexes with calsequestrin, triadin, and the ryanodine receptor, and have been shown to regulate calcium release from the sarcoplasmic reticulum. Some isoforms have been implicated in metastasis.
• Source: In Vitro Cell Free System
• Species: Human
• Tag: GST
• Molecular Mass: 50.2 kDa
• Protein length: 210 amino acids
• AA Sequence: MAEDKETKHG GHKNGRKGGL SGTSFFTWFM VIALLGVWTS VAVVWFDLVD YEEVLGKLGI YDADGDGDFD VDDAKVLLEG PSGVAKRKTK AKVKELTKEE LKKEKEKPES RKESKNEERK KGKKEDVRKD KKIADADLSR KESPKGKKDR EKEKVDLEKS AKTKENRKKS TNMKDVSSKM ASRDKDDRKE SRSSTRYAHL TKGNTQKRNG
• Applications: Enzyme-linked Immunoabsorbent Assay; Western Blot (Recombinant protein); Antibody Production; Protein Array
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCl, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: ASPH aspartate beta-hydroxylase [ Homo sapiens ]
• Official Symbol: ASPH
• Synonyms: ASPH; aspartate beta-hydroxylase; aspartyl/asparaginyl beta-hydroxylase; BAH; CASQ2BP1; HAAH; humbug; JCTN; junctate; junctin; A beta H-J-J; cardiac junctin; ASP beta-hydroxylase; peptide-aspartate beta-dioxygenase; aspartyl/asparaginyl-beta-hydroxylase; AAH
• Gene ID: 444
• mRNA Refseq: NM_001164750
• Protein Refseq: NP_001158222
• MIM: 600582
• UniProt ID: Q12797