Recombinant Full Length Ashbya Gossypii Erad-Associated E3 Ubiquitin-Protein Ligase Hrd1(Hrd1) Protein, His-Tagged

• Cat.No.: RFL25216AF
• Product Overview: Recombinant Full Length Ashbya gossypii ERAD-associated E3 ubiquitin-protein ligase HRD1(HRD1) Protein (Q75CC8) (1-575aa), fused to N-terminal His tag, was expressed in E. coli.

Specification

• Species: Ashbya gossypii
• Source: E.coli
• Tag: His
• ProteinLength: Full Length (1-575)
• Form: Lyophilized powder
• AA Sequence: MPREVSWPMWAMFITATYALAGWSAYSCATSFDDPLSALFMASSGVHFVIWGNFLIVHYC LFVWAIIRVLFGQLTAIEYDHIFERLHVVLVTLASIVITMRKTYMAGHMTILFYTLCLVA HWVLRDRMDFVFQVHGTDSSLLGILCSRFMFSLLVLGMVDYKMLKFCVQNTNVDGKRHEL YLMLALSFAQLILDVLHVVLLTSLNLFEMVRSRRTRSANLVYEGGTTDDDADDEVFILEG KYIYETVFDLTITVLKVILDIIQEVFVPWSITVVYSIFVRSIKAGESFLLVYNYWKNNKK LYEKLSDVSEEQLDDTDSMCIICMDDMLPTTETTKMNRRAKMLPCGHMLHFGCLKSWMER SQTCPICRLSVFANDSNSHATTQAREQTPPDLLQERGIDEHIDVIGMQDMSVQSISLHEG TAVRRGTTGNCMNQAYDGGLLSHEERDQAGWVAFPIEFRADNKVFFNLNDSQGDRQWMAS YTSYPRQNMVNSDDPDNASESHSRIPSPSLPGSLEGTSSQVDVTVSAKDAPANACFVIAT SKLEQTKEVEHLKRKVEELESRVEELSKRIKTDQV
• Purity: Greater than 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Tris/PBS-based buffer, 6% Trehalose, pH 8.0
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Gene Information

• Gene Name: HRD1
• Synonyms: HRD1; ACL019C; ERAD-associated E3 ubiquitin-protein ligase HRD1; RING-type E3 ubiquitin transferase HRD1
• UniProt ID: Q75CC8