Active Recombinant E.coli glk Protein, His-tagged

• Cat.No.: glk-388E
• Product Overview: Recombinant E.coli glk protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Species: E.coli
• Source: E.coli
• Tag: His
• Description: glk belongs to the bacterial glucokinase family. This protein is not highly important in E.coli as glucose is transported into the cell by the PTS system already as glucose 6-phosphate.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol.
• Bio-activity: Specific activity is >70 units/mg obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NADP. One unit will oxidize 1.0 umole of Glucose to D-glucose 6-phosphate per minute in the presence of Beta-NADP at pH
• Molecular Mass: 37.1kDa (344aa), confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMTKYALV GDVGGTNARL ALCDIASGEI SQAKTYSGLD YPSLEAVIRV YLEEHKVEVK DGCIAIACPITGDWVAMTNH TWAFSIAEMK KNLGFSHLEI INDFTAVSMA IPMLKKEHLI QFGGAEPVEG KPIAVYGAGT GLGVAHLVHV DKRWVSLPGE GGHVDFAPNS EEEAIILEIL RAEIGHVSAE RVLSGPGLVN LYRAIVKADN RLPENLKPKD ITERALADSC TDCRRALSLF CVIMGRFGGNLALNLGTFGG VFIAGGIVPR FLEFFKASGF RAAFEDKGRF KEYVHDIPVY LIVHDNPGLL GSGAHLRQTL GHIL
• Purity: >95% by SDS - PAGE
• Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 1mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: glk glucokinase [ Escherichia coli str. K-12 substr. MG1655 ]
• Official Symbol: glk
• Synonyms: ECK2384; JW2385; glucokinase
• Gene ID: 946858
• Protein Refseq: NP_416889
• UniProt ID: P0A6V8
• Pathway: Amino sugar and nucleotide sugar metabolism, organism-specific biosystem; Biosynthesis of antibiotics, organism-specific biosystem; Carbon metabolism, conserved biosystem