Recombinant E. coli RuvC

• Cat.No.: ruvC-133E
• Product Overview: Full-length recombinant protein expressed in E.coli and highly purified by combined chromatography

Specification

• Species: E.coli
• Source: E.coli
• Tag: Non
• Description: E. coli RuvC protein (19 kDa) is a structurally specific endonuclease which binds specifically to the Holliday structure, an intermediate of recombination, at the late stage of homologous recombination and recombination repair and introduces a nick in the symmetrical point of the Holliday junction cleaving and resolving the recombinant. Functional form is dimmer.
• Form: 50% glycerol, 10 mM Tris-HCl (pH7.5), 2 mM EDTA, 100 mM NaCl, 5 mM mercaptoethanol
• Purity: Over 90% by SDS-PAGE (CBB staining)
• Applications: 1) Functional studies in vitro. RuvC cleaves recombination intermediate at Holliday Junction. 2) SDS-PAGE3) Standard antigen for western blotting and ELISA
• Storage: Sent at 4℃or at -20℃ and upon arrival, centrifuge briefly and store at -20℃ or -80℃ for longer period.
• Concentration: 1.0 mg/ml (determined by BCA method)