Recombinant Dog LIF protein, His & T7-tagged

• Cat.No.: LIF-677D
• Product Overview: Recombinant Dog LIF aa. (Leu25~Ala196 (Accession # J9NRL6)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Species: Dog
• Source: E.coli
• Tag: His&T7
• Protein Length: Leu25~Ala196
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 23.8kDa
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 9.5.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH 7.4, containing 5% sucrose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: LIF LIF, interleukin 6 family cytokine [ Canis lupus familiaris (dog) ]
• Official Symbol: LIF
• Synonyms: leukemia inhibitory factor (cholinergic differentiation factor); leukemia inhibitory factor
• Gene ID: 403449
• mRNA Refseq: NM_001197073.1
• Protein Refseq: NP_001184002.1
• UniProt ID: J9NRL6