Active Recombinant Mouse Lif Protein

• Cat.No.: Lif-123M
• Product Overview: Recombinant protein of Mus musculus leukemia inhibitory factor (Lif) (Ser24-Phe203) without tag was expressed in E.coli.

Specification

• Species: Mouse
• Source: E.coli
• Protein Length: 24-203 a.a.
• Description: LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.
• Bio-activity: Measured by its ability to induce differentiation of M1 cells. ED50 for this effect is typically 0.20-0.30 ng/ml.
• Molecular Mass: 19.9 kDa
• Endotoxin: < 0.1 EU per 1 µg of the protein by the LAL
• Purity: >95%, as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for at least 6 months from date of receipt under proper storage and handling conditions.
• Storage: Store at -80 centigrade.
• Storage Buffer: Lyophilized from a sterile solution containing 20 mM phosphate buffer, pH7.4.
• Reconstitution: Resuspend the protein in the desired concentration in proper buffer.

Gene Information

• Gene Name: Lif leukemia inhibitory factor [ Mus musculus (house mouse) ]
• Official Symbol: Lif
• Synonyms: Lif; leukemia inhibitory factor; leukemia inhibitory factor; d factor; differentiation-stimulating factor; myeloid leukaemia inhibitory factor
• Gene ID: 16878
• mRNA Refseq: NM_008501
• Protein Refseq: NP_032527
• UniProt ID: P09056