Recombinant Danio rerio (zebrafish) VEGFA protein(Met1-Arg188)
Cat.No. : | VEGFA-049Z |
Product Overview : | Recombinant Zebrafish VEGF165 (O73682-Isoform VEGF165) (Met 1-Arg188) was expressed and purified in Insect Cells. |
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Species : | Zebrafish |
Source : | Insect Cells |
Tag : | Non |
Protein Length : | Met1-Arg188 |
Form : | Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
Bio-activity : | Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is typically 30-250ng/mL. |
Molecular Mass : | The recombinant zebrafish VEGF165 consists of 165 amino acids and has a calculated molecular mass of 19.2 kDa. It migrates as an 22 KDa band in SDS-PAGE under reducing conditions. |
Endotoxin : | < 1.0 EU per μg of the protein as determined by the LAL method |
Purity : | > 90 % as determined by SDS-PAGE |
Storage : | Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
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Not For Human Consumption!
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