Recombinant Cynomolgus MPL protein, His-tagged

• Cat.No.: MPL-1444C
• Product Overview: Recombinant Cynomolgus MPL protein(A0A2K5UGC7)(Gln22-Thr489), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Cynomolgus
• Tag: C-His
• Protein length: Gln22-Thr489
• Form: Supplied as 0.22μm filtered solution in 20mM Tris, 500mM NaCl, 3 mM DTT, 10% glycerol (pH 7.5).
• Bio-activity: Immobilized Cynomolgus c-MPL, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-C-MPL Antibody, hFc Tag with the EC50 of 2.3ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 53.48 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC.
• Storage: Valid for 12 months from date of receipt when stored at -80°C.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.