Recombinant Cynomolgus LRRC32 & TGFB1 protein, His-Avi-tagged, Biotinylated

• Cat.No.: LRRC32?& TGFB1-1211C
• Product Overview: Biotinylated Recombinant Cynomolgus LRRC32 & TGFB1 protein(A0A2K5X2X9(GARP)&A0A2K5TJB2(Latent TGF beta 1))(Ala18-Leu628(LRRC32) & Leu30-Ser390(TGFB1)), fused with C-terminal His and Avi tag, was expressed in HEK293.

Specification

• Species: Cynomolgus
• Source: HEK293
• Tag: His&Avi
• ProteinLength: Ala18-Leu628(LRRC32) & Leu30-Ser390(TGFB1)
• Tag: C-His-Avi
• Conjugation/Label: Biotin
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Biotinylated Cynomolgus GARP&Latent TGF Beta 1 Complex, His Tag at 2μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-GARP&TGF beta 1 Antibody, hFc Tag with the EC50 of 6.6ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 70.5 kDa (GARP)&41.3 kDa (Latent TGF Beta 1). Due to glycosylation, the protein migrates to 70-80 kDa (GARP)&13 kDa&42-50 kDa (Latent TGF Beta) based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Official Symbol: LRRC32
• Official Symbol 2: TGFB1