Native Bovine Pancreas Carboxypeptidase A, Type II-PMSF treated

• Cat.No.: CPA-01B
• Product Overview: Native Bovine Pancreas Carboxypeptidase A (Type II-PMSF treated) was purified from bovine pancreas.

Specification

• Species: Bovine
• Source: Bovine Pancreas
• Tag: Non
• Description: Carboxypeptidase as isolated from bovine pancreas glands is a metalloenzyme that contains 1 g atom of zinc per mole of protein. It catalyzes the hydrolysis of the carboxyl-terminal peptide bond in peptides and proteins. It is primarily specific to aromatic and hydrophobic side chains such as phenylalanine, tryptophan or leucine. The enzyme also exhibits esterase activity. It is inhibited by beta-phenylpropionate and indole acetate.
• Tag: Non
• Form: Ready-to-use solution
• Bio-activity: ≥50 units/mg protein
• Molecular Mass: ~35 kDa
• Impurities: ≤0.05 BTEE units/mg protein chymotrypsin
  ≤10 BAEE units/mg protein trypsin
• Grade: Proteomics Grade
• Unit Definition: One unit will hydrolyze 1.0 μmole of hippuryl-L-phenylalanine per min at pH 7.5 at 25 centigrade.
• Applications: Carboxypeptidase A from bovine pancreas has been used in a study to investigate the expression of a soluble and activatable form of bovine procarboxypeptidase A in Escherichia coli. Carboxypeptidase A from bovine pancreas has also been used in a study to investigate the isolation and partial characterization of precursor forms of ostrich carboxypeptidase.
  The enzyme has been used as a comparison to study the specificity of Metarhizium anisopliae carboxypeptidase A (MeCPA). MeCPA had been genetically engineered to facilitate the removal of polyhistidine tags from the C-termini of recombinant proteins. It has also been used to de-tyrosinate α-tubulin, in vitro, in order to induce high affinity to ethyl-N-phenylcarbamate (EPC) sepharose.
• Storage: 2-8 centigrade

Gene Information

• Official Symbol: Carboxypeptidase A
• Synonyms: Carboxypolypeptidase; Peptidyl-L-amino-acid hydrolase; 11075-17-5; 3.4.17.1 (BRENDA, IUBMB); MFCD00130718; 12352204; NA.54