Recombinant Cynomolgus IFNAR2 protein, His-tagged

• Cat.No.: IFNAR2-1417C
• Product Overview: Recombinant Cynomolgus IFNAR2 protein(EHH53981.1)(Ile27-Lys243), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Cynomolgus
• Tag: C-His
• Protein length: Ile27-Lys243
• Form: Supplied as 0.22μm filtered solution in PBS (pH 7.4).
• Bio-activity: Cynomolgus IFN alpha/beta R2, His Tag captured on CM5 Chip via anti-his antibody can bind Human IFN alpha 1, hFc Tag with an affinity constant of 70.28 nM as determined in SPR assay (Biacore T200).
• Molecular Mass: The protein has a predicted MW of 25.93 kDa. Due to glycosylation, the protein migrates to 43-60 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC.
• Storage: Valid for 12 months from date of receipt when stored at -80°C.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.