Recombinant Cynomolgus CD3E&CD3D, His & flag-tagged

• Cat.No.: CD3E&CD3D-187C
• Product Overview: Recombinant Cynomolgus CD3E & CD3D heterodimer protein (Cynomolgus CD3E & CD3D heterodimer) Gln 22 - Asp 117 (CD3E,Q95LI5) & Phe 22 - Ala 105 (CD3D,Q95LI8) was produced in human 293 cells (HEK293).

Specification

• Species: Cynomolgus
• Source: HEK293
• Tag: Flag&His
• Protein Length: 22-117;22-105 a.a.
• Description: T-cell surface glycoprotein CD3 delta & CD3 epsilon chain, also known as CD3D & CD3E or CD3D&CD3E respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.
• Predicted N Terminal: Gln 22 (CD3E) and Phe 22 (CD3D)
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.
• Molecular Mass: The heterodimer Cynomolgus CD3E & CD3D heterodimer is produced by co-expression of CD3E and CD3D, has a calculated MW of 15.5 kDa (CD3E) and 14.5 kDa (CD3D). Subunit CD3E is fused with his tag at the C-terminus and subunit CD3D is fused with flag tag at t
• Endotoxin: Less than 1.0 EU per μg of the Cynomolgus CD3E & CD3D heterodimer by the LAL method.
• Purity: >92% as determined by SDS-PAGE.
• Applications: CD Antigens
• Storage: Avoid repeated freeze-thaw cycles.No activity loss was observed after storage at:In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).

Gene Information

• Gene Name: CD3E?CD3e molecule, epsilon (CD3-TCR complex) [?Macaca fascicularis?(crab-eating macaque) ]
• Official Symbol: CD3E
• Synonyms: CD3E; CD3e molecule, epsilon (CD3-TCR complex); T-cell surface glycoprotein CD3 epsilon chain; CD3 epsilon FN18+; CD3 epsilon FN18-
• Gene ID: 102133065
• mRNA Refseq: NM_001283615
• Protein Refseq: NP_001270544
• UniProt ID: Q95LI5
• Chromosome Location: 14
• Pathway: Chagas disease (American trypanosomiasis); Hematopoietic cell lineage; Primary immunodeficiency