Active Recombinant Mouse CD3E & CD3D protein, Fc-His &Fc-Flag-tagged

• Cat.No.: CD3E & CD3D-2966M
• Product Overview: Recombinant Mouse CD3E&CD3D protein(Asp 23-Asp 108 (CD3E) & Phe 22-Ala 105 (CD3D)), fused with Fc-His and Fc-Flag tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His&Fc&Flag
• ProteinLength: Asp 23-Asp 108 (CD3E) & Phe 22-Ala 105 (CD3D)
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100mM Glycine, 25mM Arginine, 150mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Biotinylated CD3e Monoclonal Antibody at 1 μg/mL (100 μL/well) on streptavidin (0.5 μg/well) plate, can bind Mouse CD3E&CD3D Heterodimer Protein, Fc,His Tag&Fc,Flag Tag with a linear range of 0.016-0.25 μg/mL.
• Molecular Mass: Mouse CD3E&CD3D Heterodimer Protein, Fc, His Tag&Fc, Flag Tag is produced by co-expression of CD3E and CD3D, has a calculated MW of 41.5 kDa (CD3E) and 40.7 kDa (CD3D). Subunit CD3E is fused with a human IgG1 Fc tag and a polyhistidine tag at the C-terminus and subunit CD3D is fused with a human IgG1 Fc tag and a flag tag at the C-terminus. As a result of glycosylation, the heterodimer protein migrates as 55-65 kDa under reducing (R) condition, and 90-116 kDa under non-reducing (NR) condition (SDS-PAGE).
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.>90% as determined by SEC-MALS.
• Storage: For long term storage, the product should be stored at lyophilized state at-20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at:-20°C to-70°C for 12 months in lyophilized state;-70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Cd3e CD3 antigen, epsilon polypeptide [ Mus musculus ]
• Official Symbol: CD3E
• Synonyms: CD3E; CD3 antigen, epsilon polypeptide; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4 epsilon chain; CD3; T3e; AI504783; CD3epsilon
• Gene ID: 12501
• mRNA Refseq: NM_007648
• Protein Refseq: NP_031674