Active Recombinant Cynomolgus CD3E & CD3D protein, Fc-His &Fc-Flag-tagged

• Cat.No.: CD3E & CD3D-2968C
• Product Overview: Recombinant Cynomolgus CD3E&CD3D protein(Gln 22-Asp 117 (CD3E) & Phe 22-Ala 105 (CD3D)), fused with Fc-His and Fc-Flag tag, was expressed in HEK293.

Specification

• Species: Cynomolgus
• Source: HEK293
• Tag: Fc&Flag&His
• Protein Length: Gln 22-Asp 117 (CD3E) & Phe 22-Ala 105 (CD3D)
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Bio-activity: Immobilized Bispecific CD3×BCMA T cell-engaging Antibody at 1 μg/mL (100 μL/well) can bind Cynomolgus CD3E&CD3D Heterodimer Protein, Fc,His Tag&Fc,Flag Tag with a linear range of 1-20 ng/mL.
• Molecular Mass: Cynomolgus CD3E&CD3D Heterodimer Protein, Fc,His Tag&Fc,Flag Tag is produced by co-expression of CD3E and CD3D, has a calculated MW of 42.5 kDa (CD3E) and 40.9 kDa (CD3D). Subunit CD3E is fused with a human IgG1 Fc tag and a polyhistidine tag at the C-terminus and subunit CD3D is fused with a human IgG1 Fc tag and a flag tag at the C-terminus. The predicted N-terminus is Gln 22 (CD3E) & Phe 22 (CD3D). The reducing (R) protein migrates as 46-50 kDa and 55-65 kDa due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE. >90% as determined by SEC-MALS.
• Storage: For long term storage, the product should be stored at lyophilized state at-20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at:-20°C to-70°C for 12 months in lyophilized state;-70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.