Recombinant Common cattle grub HB protein, His&Myc-tagged

• Cat.No.: HB-3968C
• Product Overview: Recombinant Common cattle grub HB protein(P35588)(31-256aa), fused to N-terminal His tag and C-terminal Myc tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Common cattle grub
• Tag: His&Myc
• Form: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
  If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
• Molecular Mass: 32.1 kDa
• Protein length: 31-256aa
• AA Sequence: IVGGFEADIEDFPWQVSIQRGGYHFCGGSIYSPEIIVTAAHCLEKIDASQLRVRVGGSYWDEEGSLLTVSNFKIHEKYDAMIMWYDVALLKLSSKLTYGATVKNIELAKETPPDNADAVVSGWGTIYENYPYMPVQLRSVDVKIVSREVCRSDEYGYGNAIGPTMICAYAVGKDACQGDSGGPLVVGEALVGVVSWGEGCAYPGFPGVYTDVSVVRSWITENAKSF
• Purity: Greater than 85% as determined by SDS-PAGE.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%.

Case Study

Case 1: Lecroisey A, et al. Eur J Biochem. 1983

Hypodermin B, a serine proteinase with a molecular weight of 23000, was purified to homogeneity from the larvae Hypoderma lineatum. It is stoichiometrically inhibited by diisopropylfluorophosphate and fully inactivated by N-tosyllysine chloromethyl ketone and soya bean and bovine pancreatic trypsin inhibitors. N-Tosylphenylalanine chloromethyl ketone and ovomucoid are without effect on its activity. Hypodermin B hydrolyses both amide and ester substrates of trypsin but does not display any chymotryptic activity on synthetic substrates. Its specificity on the B chain of insulin is slightly broader than that of bovine trypsin. Its amino acid composition and N-terminal sequence suggest structural homology with serine proteinases of the trypsin family and with two other serine proteinases, hypodermin A and Hypoderma collagenase, previously isolated from the same larvae. Hypodermins A and B are very similar with respect to their inhibition and specificity, they differ however strongly from Hypoderma collagenase.

Fig1. Polyacrylamide slab gel electrophoresis at p H 8.9 and lane 3 is purified hypodermin B.

Fig2. Activity of hypodermin B, hypodermin A and Hypoderma col-lagenase at different p H values.

Application

Hypodermin B (HB) protein is a protein isolated from the common bovine Hypoderma lineatum, and its application is mainly focused on the following aspects:

Vaccine development: Hypodermin B, as one of the enzymes secreted by the first larval stage of Hypoderma lineatum, is being considered for the development of a vaccine against bovine maggot infestation. By using Hypodermin B or other related proteins as antigens, an immune response from the host can be stimulated, thus providing protection against bovine maggots.

Immunological studies: Hypodermin B plays a role in studying the immune response of cattle to Hypoderma lineatum infection. It is used in Western blotting analysis to study antibody responses in bovine serum, which helps to understand the immune status of cattle after natural infection or vaccination.

Epidemiological investigation: When studying the epidemiology of Hypoderma lineatum, Hypodermin B can be used as a marker for serological tests to help assess the infection rate and immune status of herds in different regions.

Development of diagnostic tools: Hypodermin B can be integrated into diagnostic tools such as enzyme-linked immunosorbent assay (ELISA) to detect specific antibodies in bovine serum, which is useful for early diagnosis and disease surveillance.