Recombinant Collinsella aerofaciens COLAER_02088 Protein (T189V, V207M), GST-tagged

• Cat.No.: COLAER_02088-1501C
• Product Overview: Recombinant Collinsella aerofaciens N-GST-3C-7β HSDH(T189V,V207M) protein with a GST tag was expressed in E. coli

Specification

• Species: Collinsella aerofaciens
• Source: E.coli
• Tag: GST
• ProteinLength: T189V, V207M
• Description: 7beta-hydroxysteroid dehydrogenase that catalyzes the reduction of the 7-oxo group of 7-oxo-lithocholate (7-oxo-LCA), to yield ursodeoxycholate (UDCA). As R.gnavus is a common core bacterium of the human gut microbiota, this enzyme contributes to the formation of UDCA in the human colon. UDCA is regarded as a chemopreventive beneficial secondary bile acid due to its low hydrophobicity; it protects hepatocytes and bile duct epithelial cells against necrosis and apoptosis induced by more hydrophobic secondary bile acids like deoxycholate (DCA). This enzyme is also able to catalyze the reverse reaction in vitro, i.e. the oxidation of the 7beta-hydroxy group of UDCA to 7-oxo-LCA, but much less efficiently than the reduction reaction.
• Form: Liquid
• Endotoxin: < 0.01 EU/μg of the protein
• Purity: 90%
• Stability: Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade
• Storage: Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Supplied as sterile 50 mM Tris-HCl (pH7.5), 200 mM NaCl, 20% glycerol
• Shipping: It is shipped out with blue ice.

Gene Information

• Official Symbol: 7beta-hydroxysteroid dehydrogenase
• Synonyms: 7beta-hydroxysteroid dehydrogenase
• UniProt ID: A4ECA9