Recombinant Canine NRG1 protein(Ser19-Lys240), hFc-tagged

• Cat.No.: NRG1-2342C
• Product Overview: Recombinant Canine NRG1 (XP_858147.1)(Ser19-Lys240) was expressed in HEK293 with the Fc region of Human IgG1 at the C-terminus.

Specification

• Species: Canine
• Source: HEK293
• Tag: Fc
• Protein Length: Ser19-Lys240
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured in a serum-free cell proliferation assay using MCF‑7 human breast cancer cells. The ED50 for this effect is typically 2-12 μg/mL.
• Molecular Mass: The recombinant canine NRG1/Fc is a disulfide-linked homodimer. The reduced monomer comprises 460 amino acids and has a predicted molecular mass of 50.8 kDa. The apparent molecular mass of the protein is approximately 67 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.