Recombinant Canine CD40LG Protein, Fc-tagged, Alexa Fluor 555 conjugated

• Cat.No.: CD40LG-167CAF555
• Product Overview: A DNA sequence encoding the canine CD40LG (O97626) (Met112-Leu260) (Alexa Fluor 555 conjugated) was expressed with the Fc region of mouse IgG1 at the N-terminus.

Specification

• Species: Dog
• Source: HEK293
• Tag: Fc
• Protein Length: Met112-Leu260, 385
• Form: Lyophilized
• Molecular Mass: The recombinant canine CD40LG is a disulfide-linked homodimer. The reduced monomer comprises 385 amino acids and has a predicted molecular mass of 42.5 kDa. The apparent molecular mass of the protein is approximately 47 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU/ μg of the protein as determined by the LAL method.
• Purity: > 85 % as determined by SDS-PAGE
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 555 via amines
  With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters.
• Stability: Samples are stable for up to 12 months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from sterile PBS, pH7.4.

Gene Information

• Gene Name: CD40LG CD40 ligand [ Canis lupus familiaris ]
• Official Symbol: CD40LG
• Synonyms: CD40LG; CD40 ligand; CD40-L; tumor necrosis factor ligand superfamily member 5; tumor necrosis factor (ligand) superfamily, member 5; CD40 ligand (TNF superfamily, member 5, hyper-IgM syndrome); CD154; TNFSF5
• Gene ID: 403468
• mRNA Refseq: NM_001002981
• Protein Refseq: NP_001002981