Recombinant Human CD40LG protein, His-Flag-tagged, Biotinylated

• Cat.No.: CD40LG-102HB
• Product Overview: Biotinylated Human CD40L/CD154 is expressed from mammalian with a His tag and Flag at the Nterminal. It contains M113-L261 Trimer design.

Specification

• Species: Human
• Source: HEK293
• Tag: Flag&His
• Protein Length: It contains M113-L261 Trimer design.
• Description: CD40 ligand or CD40L, also called CD154, is a protein that is primarily expressed on activated T cells and is a member of the TNF superfamily of molecules. It binds to CD40 (protein) on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In total CD40L has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD154 acts as a costimulatory molecule and is particularly important on a subset of T cells called T follicular helper cells.
• Form: Supplied as 0.22um filtered solution in PBS (pH 7.4).
• Molecular Mass: The protein has a predicted MW of 52.4 kDa, Due to glycosylation, the protein migrates to 55KDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.05EU per ug by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
• Storage: The product should be stored at -70°C. Please do not repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: CD40LG CD40 ligand [ Homo sapiens (human) ]
• Official Symbol: CD40LG
• Synonyms: CD40L; CD40-L; CD40LG; CD154 antigen; CD154; TNFSF5; TNFSF5IMD3; CD40 antigen ligand; CD40 Ligand; CD40LIGM; gp39; hCD40L; HIGM1; T-B cell-activating molecule; T-BAM; T-cell antigen Gp39; TNF-related activation protein; TRAP; TRAPtumor necrosis factor (ligand) superfamily, member 5 (hyper-IgM syndrome); tumor necrosis factor (ligand) superfamily member 5; Tumor necrosis factor ligand superfamily member 5
• Gene ID: 959
• mRNA Refseq: NM_000074.3
• Protein Refseq: NP_000065.1
• MIM: 300386
• UniProt ID: P29965