Recombinant Bovine CXCL5 protein

• Cat.No.: CXCL5-42B
• Product Overview: Recombinant Bovine CXCL5 protein was expressed in E.coli.

Specification

• Species: Bovine
• Source: E.coli
• Tag: Non
• Protein Length: 76
• Description: GCP-2 (granulocyte chemotactic protein-2) is a CXC chemokine. Among human CXC chemokines, GCP-2 is most closely related to ENA-78. The structure and sequence of the genes for human GCP-2 and ENA-78 also exhibit close similarity, suggesting the two genes may have originated from a recent gene duplication. LIX (LPS-induced CXC chemokine) was initially cloned as a gene induced by LPS in mouse fibroblasts. The mouse protein designated GCP-2, because of its amino acid sequence similarity (60%) to human GCP-2, is identical to the LIX protein sequence.
• Form: Lyophilized from a 0.2 µm filtered concentrated solution in 20 mM PB, 500 mM NaCl, pH 7.0.
• Bio-activity: Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration range of 10-50 ng/ml.
• Molecular Mass: Approximately 8.0 kDa, a single non-glycosylated polypeptide chain containing 76 amino acids.
• AA Sequence: GPVAAVVRELRCVCLTTTPGIHPKTVSDLQVIAAGPQCSKVEVIATLKNGREVCLDPEAPLIKKIVQKILDSGKNN
• Endotoxin: Less than 0.1 EU/µg of rBoGCP-2/CXCL6 as determined by LAL method.
• Purity: >97% by SDS-PAGE and HPLC analyses.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 centigrade. Further dilutions should be made in appropriate buffered solutions.

Gene Information

• Gene Name: CXCL5
• Official Symbol: CXCL5
• Synonyms: GCP2; CXCL6; GCP-2; SCYB6
• Gene ID: 281735
• mRNA Refseq: NM_174300.2
• Protein Refseq: NP_776725.1
• UniProt ID: P80221