Active Recombinant Mouse Cxcl5 Protein (74 aa)

• Cat.No.: Cxcl5-228C
• Product Overview: Recombinant mouse LIX/CXCL5 (74aa) produced in CHO cells is a single polypeptide chain containing 74 amino acids. rmLIX/CXCL5(74aa) has a molecular mass of 8 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques.

Specification

• Species: Mouse
• Source: CHO
• Protein Length: 74
• Description: Mouse LIX (C-X-C motif chemokine 5) is a small cytokine belonging to the CXC chemokine family that is cleaved into the following 2 chains [GCP-2(1-78) and GCP-2(9-78)]. Mouse LIX plays a role in reducing sensitivity to sunburn pain in some subjects, and is a potential target which could be used to understand more about pain in other inflammatory conditions. It is most closely related to two highly homologous human neutrophil chemoattractants GCP-2 and ENA-78. The first 78 amino acid residues within the predicted mature mouse LIX shares approximately 61% and 55% amino acid identity with human GCP-2 and ENA-78. This chemokine stimulates the chemotaxis of neutrophils possessing angiogenic properties. It elicits these effects by interacting with the cell surface chemokine receptor CXCR2.
• Form: Sterile Filtered White lyophilized (freeze-dried) powder.
• Bio-activity: The EC50 value of mouse LIX/CXCL5 (74aa) on Ca^2+ mobilization assay in CHO-K1/Gα15/mCXCR2 cells (human Gα15 and mouse CXCR2 stably expressed in CHO-K1 cells) is less than 200 ng/mL.
• Molecular Mass: 8 kDa, observed by reducing SDS-PAGE.
• AA Sequence: VIAATELRCVCLTVTPKINPKLIANLEVIPAGPQCPTVEVIAKLKNQKEVCLDPEAPVIKKIIQKILGSDKKKA
• Endotoxin: < 0.2 EU/μg, determined by LAL method.
• Purity: > 98% as analyzed by SDS-PAGE.
• Storage: Lyophilized recombinant mouse LIX/CXCL5 remains stable up to 6 months at -80 centigrade from date of receipt. Upon reconstitution,mouse LIX/CXCL5 should be stable up to 1 week at 4 centigrade or up to 2 months at -20 centigrade.
• Storage Buffer: Lyophilized after extensive dialysis against PBS.
• Reconstitution: Reconstituted in ddH2O or PBS at 100 μg/mL.

Gene Information

• Gene Name: Cxcl5 chemokine (C-X-C motif) ligand 5 [ Mus musculus ]
• Official Symbol: Cxcl5
• Synonyms: CXCL5; chemokine (C-X-C motif) ligand 5; C-X-C motif chemokine 5; cytokine LIX; small-inducible cytokine B5; granulocyte chemotactic protein-2; small inducible cytokine B subfamily, member 5; small inducible cytokine subfamily B, member 15; LIX; GCP-2; Scyb5; Scyb6; ENA-78; AMCF-II
• Gene ID: 20311
• mRNA Refseq: NM_009141
• Protein Refseq: NP_033167
• UniProt ID: P50228