Recombinant B. burgdorferi ospC Antigen, His tagged

• Cat.No.: ospC-039B
• Product Overview: The Borrelia burgdorferi antigen ospC has been prepared as a recombinant antigen fused to a his-tag in its N-terminus.

Specification

• Species: B. burgdorferi
• Source: E.coli
• Tag: His
• Description: Outer membrane protein ospC is essential for the invasive strategy of the pathogenic spirochete responsible for Lyme disease. Detection of anti-OspC borreliacidal antibodies, especially IgM antibodies, in early Lyme borreliosis sera provides additional evidence that borreliacidal antibody detection may be useful for the serodiagnosis of early Lyme borreliosis.
• Tag: N-His
• Molecular Mass: Determined by SDS-PAGE, the protein band is between molecular markers of 25 and 35 kDa, while relative molecular mass calculated from amino acid sequence is 27,887.3 Da.
• Purity: > 95% by observation on SDS-PAGE electrophoresis
• Applications: WB, DB, IE, DE, CLIA, LF
• Storage: Upon arrival, it should be aliquoted to avoid repeated freezing and thawing cycles and stored at -20 to -80 centigrade. In order to defrost the protein, maintain the aliquot at 25 centigrade without shaking to avoid aggregation.
• Storage Buffer: 20 mM phosphate buffer pH 7 and 0.15 M NaCl
• Concentration: 1.91 mg/mL
• Shipping: Protein is shipped with dry ice.
• Reference: 1. Lagal, V., le Postic, D., Ruzic-Sabljic, E., and G. Baranton. Genetic Diversity among Borrelia Strains Determined by Single-Strand Conformation Polymorphism Analysis of the ospC Gene and Its Association with Invasiveness. 2003. Journal of Clinical Microbiology, 41:5059–5065.
  2. Bradford, MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal Biochem. 1976, 131:499-503.