Recombinant B. afzelii ospC Antigen, His&TrxA tagged

• Cat.No.: ospC-041B
• Product Overview: The Borrelia afzelii antigen ospC has been prepared as a recombinant antigen fused to the thioredoxine protein and a his-tag in its N-terminus. It is produced from the complete ORF of the gene of the outer surface protein C.

Specification

• Species: B.afzelii
• Source: E.coli
• Tag: His&TRxA
• Description: Outer membrane protein ospC is essential for the invasive strategy of the pathogenic spirochete responsible for Lyme disease. Detection of anti-OspC borreliacidal antibodies, especially IgM antibodies, in early Lyme borreliosis sera provides additional evidence that borreliacidal antibody detection may be useful for the serodiagnosis of early Lyme borreliosis.
• Tag: N-His&TrxA
• Molecular Mass: Determined by SDS-PAGE, the protein band is between molecular markers of 35 and 45 kDa, while relative molecular mass calculated from amino acid sequence is 39,224.30 Da.
• Purity: > 95% by observation on SDS-PAGE electrophoresis
• Applications: WB, DB, IE, DE, CLIA, LF
• Storage: Upon arrival, it should be aliquoted to avoid repeated freezing and thawing cycles and stored at -20 to -80 centigrade. In order to defrost the protein, maintain the aliquot at 25 centigrade without shaking to avoid aggregation.
• Storage Buffer: 20 mM phosphate buffer pH 8, 0.15 M NaCl and 0,1% polyoxyethylene (10) tridecylether
• Concentration: 3.71 mg/mL
• Shipping: Protein is shipped with dry ice.
• Reference: 1. Lagal, V., le Postic, D., Ruzic-Sabljic, E., and G. Baranton. Genetic Diversity among Borrelia Strains Determined by Single-Strand Conformation Polymorphism Analysis of the ospC Gene and Its Association with Invasiveness. 2003. Journal of Clinical Microbiology, 41:5059–5065.
  2. Gill SC, von Hippel PH. Calculation of protein extinction coefficients from amino acid sequence data. Anal Biochem. 1989 Nov 1;182(2):319-26.