Recombinant B. burgdorferi Flagellin Protein, MBP-tagged

• Cat.No.: Flagellin-14B
• Product Overview: Recombinant Borrelia burgdorferi Flagellin protein (NCBI accession number ZP_03087263.1) produced in E. coli. Protein contains a MBP-tag and is visible on SDS-PAGE at 76.3 kDa Flagellin-MBP (33.9 kDa for Flagellin and 42.4 kDa for MBP)

Specification

• Species: Borrelia Burgdorferi
• Source: E.coli
• Tag: MBP
• Description: Flagellin is encoded by the spirochete B. burgdorferi, which is carried by Ixodes ticks. Strain ZS7 was isolated from Ixodes rizinus ticks collected by flagging in the Freiburg, Germany area in the 1980s. The flagella of B. burgdorferi are different from those of other spirochaetes in that they are not surrounded by an outer sheath layer but are comprised of a single flagellin protein. Unlike other bacterial flagella, those of the spirochaetes are situated within the periplasm and are referred to as periplasmic flagella. Studies comparing aflagellate Borrelia to flagellated indicate that the flagella have a role in the invasion of human tissue.
• Form: Liquid, 0.2 μm filter sterilised.
• Molecular Mass: 76.3 kDa for Flagellin-MBP (33.9 kDa for Flagellin and 42.4 kDa for MBP)
• Purity: > 90%
• Storage: Short term: +2 to +8 centigrade
  Long Term: -80 centigrade
  Can be frozen, but avoid multiple freeze/thaw cycles
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Storage Buffer: In 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide.

Gene Information

• Official Symbol: Flagellin
• Synonyms: flagellin
• Protein Refseq: ZP_03087263