Recombinant B. burgdorferi CSPz Protein, MBP-tagged

• Cat.No.: CSPz-02B
• Product Overview: Recombinant Borrelia burgdorferi CRASP-2 protein produced in E. coli. Protein contains a MBP-tag and is visible on SDS-PAGE at 67.8 kDa for CRASP-2-MBP (25.4 kDa for CRASP-2 and 42.4 kDa for MBP)

Specification

• Species: Borreliella burgdorferi B31
• Source: E.coli
• Tag: MBP
• Description: CRASP-2 is encoded by the spirochete B. burgdorferi, which is carried by Ixodes ticks. Strain B31 is the type strain (ATCC 35210) for this organism and was derived by limited dilutional cloning from the original Lyme-disease tick isolate obtained by A. Barbour. CRASP-2 (CSPz) is a 20/21-kDa protein which binds to FHL-1 and factor H binding protein (although it interacts preferentially with Factor H) and may be predominantly expressed by serum-resistant Borrelia strains. It is possible that because of discontinuous binding regions in the factor H/FHL-1, a long-distance interaction may be involved in binding of both immune regulators. Putative coiled-coil structural elements may also be important in the interaction of B. burgdorferi CRASP-1 with factor H.
• Form: Liquid, 0.2 μm filter sterilised.
• Molecular Mass: 67.8 kDa for CRASP-2-MBP (25.4 kDa for CRASP-2 and 42.4 kDa for MBP)
• Purity: > 90%
• Storage: Short term: +2 to +8 centigrade
  Long Term: -80 centigrade
  Can be frozen, but avoid multiple freeze/thaw cycles
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Storage Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide.

Gene Information

• Gene Name: cspZ factor H-binding protein CspZ [ Borreliella burgdorferi B31 ]
• Official Symbol: cspZ
• Synonyms: cspZ; factor H-binding protein CspZ
• Gene ID: 1194149
• Protein Refseq: WP_010890313