Recombinant Arabidopsis Thaliana AUR1 Protein (1-294 aa), His-SUMO-Myc-tagged

• Cat.No.: AUR1-2173A
• Product Overview: Recombinant Arabidopsis Thaliana (Mouse-ear cress) AUR1 Protein (1-294 aa) is produced by E. coli expression system. This protein is fused with a 10xHis-SUMO tag at the N-terminal and a Myc tag at the C-terminal. Protein Description: Full Length.

Specification

• Species: Arabidopsis Thaliana
• Source: E.coli
• Tag: His&Myc&SUMO
• Protein Length: 1-294 aa
• Description: Phosphorylates specifically 'Ser-10' of histone H3 in vitro and colocalizes with phosphorylated histone H3 during mitosis. Associates with cytoskeletal structures that are necessary for cytokinesis and with the microtubule spindle. Colocalizes also with gamma-tubulin and function in microtubule organizing centers (MTOCs). In contrast with the mammalian B-type Aurora, AUR1 has no kinase activity toward 'Ser-28' of histone H3.
• Form: Tris-based buffer,50% glycerol
• Molecular Mass: 54.0 kDa
• AA Sequence: MAIPTETQHQEKEASDASAAAAQKRWTLSDFDIGKPLGRGKFGHVYLAREKRSNHVVALKVLFKSQLQQSQVEHQLRREVEIQSHLRHPNILRLYGYFYDQKRVYLILEYAARGELYKDLQKCKYFSERRAATYVASLARALIYCHGKHVIHRDIKPENLLIGAQGELKIADFGWSVHTFNRRRTMCGTLDYLPPEMVESVEHDASVDIWSLGILCYEFLYGVPPFEAMEHSDTYRRIVQVDLKFPPKPIISASAKDLISQMLVKESSQRLPLHKLLEHPWIVQNADPSGIYRV
• Purity: > 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA with reconstitution instruction is sent along with the products.

Gene Information

• Gene Name: AUR1 ataurora1 [ Arabidopsis thaliana (thale cress) ]
• Official Symbol: AUR1
• Synonyms: AUR1; AtAUR1; ataurora1; T16I18.40; T16I18_40
• Gene ID: 829419
• mRNA Refseq: NM_119436
• Protein Refseq: NP_195009
• UniProt ID: Q9M077