Recombinant Active Rat MMP9 Protein, His-tagged(C-ter)

• Cat.No.: Mmp9-243R
• Product Overview: Recombinant Active Rat MMP9 Protein with His tag (C-ter) was expressed in HEK293.

Specification

• Species: Rat
• Source: HEK293
• Tag: His
• Protein Length: Leu35-Pro708
• Description: Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. [provided by RefSeq, Jul 2008]
• Form: Powder
• Bio-activity: Measured in a cell migration assay using HeLa cells. 1 ng/mL of Recombinant Rat MMP-9 can effectively induce HeLa cells migration.
• Endotoxin: Endotoxin level is less than 0.1 EU/μg of the protein, as determined by the LAL test.
• Purity: > 95% (by SDS-PAGE)
• Applications: FuncSt, SDS-PAGE
• Notes: For laboratory research only, not for drug, diagnostic or other use.
• Storage: Lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C for up to one month, at 2-8°C for up to one week. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
• Storage Buffer: 50 mM Tris (pH 7.5), 10 mM CaCl2 and 150 mM NaCl.
• Reconstitution: Reconstitute to a concentration of 0.1-0.5 mg/ml in sterile distilled water.

Gene Information

• Gene Name: Mmp9 matrix metallopeptidase 9 [ Rattus norvegicus ]
• Official Symbol: Mmp9
• Synonyms: MMP9; matrix metallopeptidase 9; matrix metalloproteinase-9; GELB; MMP-9; gelatinase B; 92 kDa gelatinase; 92 kDa type IV collagenase; 92-kDa type IV collagenase; matrix metalloproteinase 9 (gelatinase B 92-kDa type IV collagenase); matrix metalloproteinase 9 (gelatinase B, 92-kDa type IV collagenase)
• Gene ID: 81687
• mRNA Refseq: NM_031055
• Protein Refseq: NP_112317