Recombinant Active Human AAK1 Protein (1-465), N-GST tagged

• Cat.No.: AAK1-893H
• Product Overview: Recombinant human AAK1 (1-465) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Protein Length: 1-465 a.a.
• Description: This gene encodes a member of the SNF1 subfamily of serine/threonine protein kinases. Adaptor-related protein complex 2 (AP-2 complexes) functions during receptor-mediated endocytosis to trigger clathrin assembly, interact with membrane-bound receptors, and recruit encodytic accessory factors. The encoded protein interacts with and phosphorylates a subunit of the AP-2 complex, which promotes binding of AP-2 to sorting signals found in membrane-bound receptors and subsequent receptor endocytosis. Its kinase activity is stimulated by clathrin. This kinase has been shown to play an important role in regulating the clathrin-mediated endocytosis of the rabies virus, facilitating infection. Inhibitors of this kinase are being studied as candidate therapeutics to disrupt the entry of viruses, including SARS-CoV-2, into target cells. It is also involved in positive regulation of Notch pathway signaling in mammals. Alternatively spliced transcript variants have been described, but their biological validity has not been determined.
• Bio-activity: 9.2 nmol/min/mg
• Molecular Mass: 78 kDa
• Purity: >80%
• Stability: 1yr at -70 centigrade from date of shipment
• Storage: Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1 μg/μL
• Storage Buffer: Recombinant protein stored in 50mM Tris-HCI, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DIT, 0.1mM PMSF, 25% glycerol.

Gene Information

• Gene Name: AAK1 AP2 associated kinase 1 [ Homo sapiens (human) ]
• Official Symbol: AAK1
• Synonyms: AAK1; AP2 associated kinase 1; AP2-associated protein kinase 1; adaptor-associated kinase 1; EC 2.7.11.1
• Gene ID: 22848
• mRNA Refseq: NM_014911
• Protein Refseq: NP_055726
• MIM: 616405
• UniProt ID: Q2M2I8