Recombinant AAV9 VP1 + VP2 + VP3 Protein, N-His-tagged

• Cat.No.: VP1/VP2/VP3-17A
• Product Overview: Recombinant AAV9 VP1/VP2/VP3 Protein with a N-His tag was expressed in E. coli and purified by Ni-NTA chromatography.

Specification

• Species: AAV9
• Source: E.coli
• Tag: His
• Description: The AAV capsid consists of three capsid proteins, i.e. VP1, VP2 and VP3, which differ in their N-terminus and encapsulate the genomic ssDNA. In native virus particles, the three proteins form subunits with a ratio of 1:1:10 (VP1:VP2:VP3), in a total number of 60 subunits per capsid.
• Form: Liquid
• Molecular Mass: VP1: ~90 kDa, VP2: ~68 kDa, VP3: ~62 kDa
• Purity: > 95% (determined by SDS-PAGE)
• Applications: Dot Blot: 100 ng, depending on primary antibody and detection method
  SDS-PAGE: 1 μg
  WB: 5-20 ng, depending on primary antibody and detection method
• Storage: At -80 centigrade.
• Concentration: 100 μg/mL (VP1: 1.19 μM, VP2: 1.45 μM, VP3: 1.61 μM)
• Storage Buffer: 6 M urea in PBS

Gene Information

• Official Symbol: VP1/VP2/VP3
• Synonyms: AAV2gp05; major coat protein VP1; AAV2gp06; assembly activating protein AAP; major coat protein VP2; AAV2gp07; major coat protein VP3; VP1/VP2/VP3; VP1; VP2; VP3