Active Recombinant SARS-CoV-2 papain-like protease Protein, His-tagged

• Cat.No.: PLpro-167S
• Product Overview: Active recombinant SARS-CoV-2 N-terminal His-tagged papain-like protease was expressed in E. coli.

Specification

• Species: SARS-CoV-2
• Source: E.coli
• Tag: His
• Protein Length: 1564-1880
• Description: Replication of severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) requires proteolytic processing of the replicase polyprotein by two viral cysteine proteases,a chymotrypsin-like protease (3CLpro) and a papain-like protease (PLpro).These proteases are important targets for development of antiviral drugs that would inhibit viral replication and reduce mortality associated with outbreaks of SARS-CoV.PLpro is a cysteine protease located within the non-structural protein 3 (NS3) section of the viral polypeptide.PLPro activity is required to process the viral polyprotein into functional,mature subunits;specifically,PLPro cleaves a site at the amino-terminus of the viral replicase region.In addition to its role in viral protein maturation,PLPro possesses a deubiquitinating and deISGylating activity.In vivo,this protease antagonizes innate immunity by inhibiting IRF3-induced production of type I interferons.
• Form: Lyophilized
• Bio-activity: >80 pmols/min/μg
• Molecular Mass: 36.8 kDa
• Purity: ≥90% estimated by SDS-PAGE
• Stability: ≥ 6 months
• Storage: At -80 centigrade.
• Storage Buffer: Lyophilized from sterile 20 mM Tris, pH 7.4, with 500 mM sodium chloride

Gene Information

• Synonyms: 2019-nCoV PLpro; COVID-19-PLpro; SARS-CoV-2 PLpro; Severe Acute Respiratory Syndrome Coronavirus 2 Papain-like Protease