Nucleosome Full Panel

• Cat.No.: NUC-065
• Product Overview: A 96-well plate containing 77 unique mononucleosomes assembled from recombinant human histones expressed in E. coli.

Specification

• Source: E.coli
• Tag: Non
• Description: A 96-well plate containing 77 unique mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3 and H4; accession numbers: H2A-P04908, H2B-O60814, H3.1-P68431/H3.2-Q71DI3/H3.3-P84243, H4-P62805). Single and combinatorial histone post-translational modifications (PTMs) are created using a proprietary synthetic method. The nucleosomes are wrapped by 147 or 187 base pairs DNA containing a central 601-positioning sequence (Lowary and Widom 1998 J Mol Biol. (1):19-42) with a 5’ biotin-TEG group.
• Form: Purified recombinant mononucleosomes individually stored in a 96 well plate (1.5 μg nucleosome at 1.5 µM per well) in 5 μL of nucleosome storage buffer (10 mM Tris-HCl pH 7.5, 1 mM EDTA, 25 mM NaCl, 2 mM DTT, & 20% glycerol).
• Molecular Mass: ~200,000 Da
• Applications: The access to epigenetic diversity in a physiological nucleosome context enables broad end-user applications, including nucleosome binding studies (e.g. chromatin reader binding preferences, see application data below for example using BRD4 BD-1), enzyme screening assays (e.g. identification of preferred substrates), and antibody specificity testing (e.g. for applications that require antibody specificity in a nucleosome, rather than histone peptide context). The biotin group on the DNA facilitates pull-down experiments. Mononucleosomes do not contain free DNA which could alter assayed activities.
• Storage: Stable for six months at -80°C from date of receipt.