Neutrophil elastase colorimetric drug discovery Kit
Cat.No. : | Kit-0323 |
Product Overview : | It is a complete assay system designed to screen inhibitors of neutrophil elastase, providing enough material to perform at least 96 assays. |
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Tag : | Non |
Size : | 96 wells |
Description : | Neutrophil elastase, also known as leukocyte elastase (LE), is a serine protease found in polymorphonuclear neutrophils (PMNs) that functions in inflammation. It can cleave a variety of proteins, including fibrous elastin, collagen, clotting factors, TNF-α, and α1-antichymotrypsin. It has been shown to be taken up by tumor cells and accelerate tumor growth through its degradation of insulin receptor substrate-1 (IRS-1). Because of its potential roles in cancer and inflammatory diseases such as emphysema, cystic fibrosis, and atherosclerosis, it is a target for drug discovery. The kit uses the chromogenic substrate MeOSuc-Ala-Ala-Pro-Val-pNA; cleavage of p-nitroaniline (pNA) from the substrate increases absorbance at 405nm. A neutrophil elastase inhibitor, elastatinal, is included for use as a control. |
Applications : | Colorimetric detection, HTS |
Storage : | -80°C |
Kit Components : | Neutrophil elastase (human), (purified)pNA substrate (MeOSuc-AAPV-pNA) (60μL; 20mM in DMSO)pNA calibration standard (p-nitroaniline) (1ml; 50μM in assay buffer)Elastatinal (inhibitor) (10μL; 20mM in DMSO)Neutrophil elastase assay buffer (20ml; 100mM HEPES, pH 7.25, 500mM NaCl, 0.05% Tween-20; liquid in a screw-cap plastic bottle)96-well microplate |
◆ Recombinant Proteins | ||
Elane-1419M | Recombinant Mouse Elane Protein, His-tagged | +Inquiry |
ELANE-3398H | Recombinant Human ELANE protein, His-SUMO-tagged | +Inquiry |
ELANE-1653H | Human Elastase | +Inquiry |
ELANE-7584H | Recombinant Human ELANE protein | +Inquiry |
Elane-694M | Active Recombinant Mouse Elane Protein, His-tagged | +Inquiry |
◆ Native Proteins | ||
ELANE-3221H | Active Native Human ELANE Protein | +Inquiry |
ELANE-001H | Active Native Human ELANE Protein | +Inquiry |
ELANE-8104H | Native Human Neutrophil Elastase | +Inquiry |
ELANE-27537TH | Native Human ELANE | +Inquiry |
ELANE-8236H | Native Human Neutrophil Elastase (ELA-2) | +Inquiry |
◆ Cell & Tissue Lysates | ||
ELANE-2381MCL | Recombinant Mouse ELANE cell lysate | +Inquiry |
Not For Human Consumption!
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