Native Clostridium histolyticum Clostripain, Sequencing Grade

• Cat.No.: Clostripain-02C
• Product Overview: Tissue total protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE analysis.

Specification

• Species: Clostridium histolyticum
• Description: Clostripain (Endoproteinase-Arg-C) is a two chain proteinase associated with collagenase and isolated from Clostridium histolyticum. It is highly specific for the carboxyl peptide bond of arginine. Clostripain has a sulfhydryl requirement; it is activated by dithiothreitol, cysteine, or other sulfhydryl containing reagents. The presence of calcium ions is essential. The enzyme is inhibited by oxidizing agents and sulfhydryl reactants and by Co2+, Cu2+, Cd2+, and heavy metal ions. Citrate, borate, and Tris anions are less inhibitory.
• Form: A dialyzed, lyophilized, pre-activated powder.
• Bio-activity: ≥50 units per mg dry weight
• CAS #: 9028-00-6
• Molecular Mass: 53.0 kDa
  Light chain: 12.5 kDa
  Heavy chain: 45 kDa
• Specificity: Clostripain selectively hydrolyzes arginyl bonds and lysyl bonds at a lower rate. It can also act as a transpeptidase with maximal activity at pH 7.6-9.0.
• Extinction Coefficient: 87,890 cm-1 M-1 (Theoretical)
  E1%,280= 16.57 (Theoretical)
• Isoelectric point: 4.8-4.9
• Optimal pH: 7.4-7.8 (activity against a-benzoyl-arginine ethyl ester)
• Activators: Sulfhydryl requirement: dithiothreitol, cysteine, or other reducing agents
  Calcium ion is essential
  Reducing agents
• Inhibitors: EDTA
  Oxidizing agents
  Sulfhydryl reagents (such as TLCK)
  Co2+, Cu2+, Cd2+, and heavy metal ions
  Citrate, borate and Tris anions partially inhibit
• Unit Definition: One Unit hydrolyzes one micromole of N-benzoyl-L-arginine ethyl ester per minute at 25 centigrade, pH 7.6, in the presence of dithiothreitol.
• Applications: Peptide mapping
  Sequence analysis
  Cell isolation
  Hydrolysis/condensation of amide bonds
  Peptide synthesis
• Storage: Store at 2-8 centigrade.

Gene Information

• Official Symbol: Clostripain
• Synonyms: Clostripain; Endoproteinase-Arg-C; CAS 9028-00-6; EC: 3.4.22.8
• UniProt ID: P09870