GMP Recombinant Rhesus macaque IL-6

• Cat.No.: IL6-22R

Specification

• Species: Rhesus macaque
• Source: E.coli
• Tag: Non
• Description: Interleukin-6 (IL-6) is a pleiotropic cytokine that plays an important role in host defense by regulating immune and inflammatory responses. Produced by T cells, monocytes, fibroblasts, endothelial cells and keratinocytes, IL-6 has diverse biological functions. It stimulates B-cell differentiation and antibody production, synergizes with IL-3 in megakaryocyte development and platelet production, induces expression of hepatic acute-phase proteins, and regulates bone metabolism. IL-6 signals through the IL-6 receptor system that consists of two chains, IL-6Rα and gp130.
• Form: Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
• Bio-activity: The ED50 determined by a cell proliferation assay usingIL-6-dependent murine 7TD1 cells is less than 0.1 ng/mL, corresponding to a specific activity of >1.0 × 107 IU/mg.
• Molecular Mass: Approximately 21.1 kDa, a single non-glycosylated polypeptide chain containing 186 amino acids.
• AA Sequence: MAPVLPGEDS KNVAAPHSQP LTSSERIDKH IRYILDGISA LRKETCNRSNMCESSKEALA ENNLNLPKMA EKDGCFQSGF NEDTCLVKII TGLLEFEVYLEYLQNRFESS EEQARAVQMS TKVLIQFLQK KAKNLDAITT PEPTTNASLLTKLQAQNQWL QDMTTHLILR SFKEFLQSNL RALRQM
• Endotoxin: Less than 1 EU/μg of IL-6 as determined by LAL method.
• Purity: >97% by SDS-PAGE and HPLC analyses.
• Usage: This material is offered by Creatvie Biomart for research, laboratory or further evaluation purposes. FOR RESEARCH USE ONLY. NOT INTENDED FOR ANY ANIMAL OR HUMAN THERAP EUTIC OR DIAGNOSTIC USE.
• Storage Buffer: This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at< -20°c.="" further="" dilutions="" should="" be="" made="" in="" appropriate="" buffered="">

Gene Information

• Gene Name: IL6 interleukin 6 (interferon, beta 2) [ Macaca mulatta ]
• Official Symbol: IL6
• Synonyms: interleukin-6; IL-6
• Gene ID: 705819
• mRNA Refseq: NM_001042733
• Protein Refseq: NP_001036198
• Chromosome Location: chromosome: 3
• Pathway: African trypanosomiasis, organism-specific biosystem; Amoebiasis, conserved biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem