| Species : |
Rhesus macaque |
| Source : |
E.coli |
| Tag : |
Non |
| Description : |
Interleukin-6 (IL-6) is a pleiotropic cytokine that plays an important role in host defense by regulating immune and inflammatory responses. Produced by T cells, monocytes, fibroblasts, endothelial cells and keratinocytes, IL-6 has diverse biological functions. It stimulates B-cell differentiation and antibody production, synergizes with IL-3 in megakaryocyte development and platelet production, induces expression of hepatic acute-phase proteins, and regulates bone metabolism. IL-6 signals through the IL-6 receptor system that consists of two chains, IL-6Rα and gp130. |
| Form : |
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
| Bio-activity : |
The ED50 determined by a cell proliferation assay usingIL-6-dependent murine 7TD1 cells is less than 0.1 ng/mL, corresponding to a specific activity of >1.0 × 107 IU/mg. |
| Molecular Mass : |
Approximately 21.1 kDa, a single non-glycosylated polypeptide chain containing 186 amino acids. |
| AA Sequence : |
MAPVLPGEDS KNVAAPHSQP LTSSERIDKH IRYILDGISA LRKETCNRSNMCESSKEALA ENNLNLPKMA EKDGCFQSGF NEDTCLVKII TGLLEFEVYLEYLQNRFESS EEQARAVQMS TKVLIQFLQK KAKNLDAITT PEPTTNASLLTKLQAQNQWL QDMTTHLILR SFKEFLQSNL RALRQM |
| Endotoxin : |
Less than 1 EU/μg of IL-6 as determined by LAL method. |
| Purity : |
>97% by SDS-PAGE and HPLC analyses. |
| Usage : |
This material is offered by Creatvie Biomart for research, laboratory or further evaluation purposes. FOR RESEARCH USE ONLY. NOT INTENDED FOR ANY ANIMAL OR HUMAN THERAP EUTIC OR DIAGNOSTIC USE. |
| Storage Buffer : |
This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles. |
| Reconstitution : |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at< -20°c.="" further="" dilutions="" should="" be="" made="" in="" appropriate="" buffered=""> |
